Qualitative Analysis of Serum Proteins in Benign Prostatic Hyperplasia Separated by Sds-page

This study was conducted for qualitative analysis of serum proteins separated by SDS-PAGE and stained by Coomassie Brilliant Blue R-250 in order to describe the preliminary identification of serum proteins that may act as diagnostic marker in benign prostatic hyperplasia. Serum samples of 23 biopsy confirmed cases of benign prostatic hyperplasia and normal controls of similar age group were subjected to SDS-PAGE on a 12% resolving gel, followed by staining with Coomassie Brilliant Blue R-250. Protein fractions were analyzed using computer software program “GeneGenius Gel Documentation and Analysis System”. Major protein fractions ranging in molecular weights from 1.45157 kDa were observed. Raw volumes of most of the protein fractions seem to be increased in majority of benign prostatic hyperplasia cases as compared to normal control. Protein fractions 55-57 kDa were undetected in normal controls under 80 years of age but appeared in 56% of benign prostatic hyperplasia cases. Two dimensional gel electrophoresis and silver staining of these samples could yield better resolution of protein fraction 55-57 kDa that could serve as marker for benign prostatic hyperplasia.